中国骨质疏松杂志

维生素D对骨质疏松大鼠模型骨量缺损的修复作用

The repair effect of vitamin D on bone mass defect in a rat model of osteoporosis

DOI：10.3969/j.issn.1006-7108.2025.11.004

英文关键词:vitamin D osteoporosis peroxisome proliferator activated receptor γ

基金项目:国家自然科学基金资助项目（82274544）；江西省卫生健康委科技计划项目（202311426）；江西省中医药管理局科技计划项目（2023B0280）；广州市科技计划项目（202206010184）；2021年广东省教育厅普通高校重点领域专项（2021ZDZX2005）

作者	单位
兰浩1 康庐琛1* 宁静1 李文斌1 魏秋实2	1.九江市第一人民医院（九江市细胞治疗重点实验室）内分泌内科，江西九江 332000 2.广州中医药大学第三附属医院中医骨关节科，广东广州 510378

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中文摘要:

目的探讨维生素D通过调控Ⅱ型胶原蛋白（Col Ⅱ）、过氧化物酶体增殖物激活受体γ（PPAR-γ）和激活蛋白2（AP-2） mRNA及蛋白的表达对骨质疏松大鼠模型骨量缺损的修复作用。方法将SD雄性大鼠随机分为假手术组、骨质疏松组、维生素D组。检测各组大鼠骨密度、股骨生物力学和骨代谢相关指标，采用反转录聚合酶链式反应（RT-PCR）法和蛋白印迹（Western blot）法检测大鼠骨组织中ColⅡ、PPAR-γ、AP-2 mRNA及蛋白表达水平。结果与假手术组比较，骨质疏松组大鼠股骨松质骨密度、皮质骨密度、总体骨密度、最大载荷、最大应力、最大能量、刚度、血清钙（Ca）水平、Col Ⅱ mRNA及蛋白表达水平均明显降低，血清骨特异性碱性磷酸酶（BALP）、磷（P）、甲状旁腺素（PTH）水平、PPAR-γ、AP-2 mRNA及蛋白表达水平均明显升高（P＜0.05）。与骨质疏松组比较，维生素D组大鼠股骨松质骨密度、皮质骨密度、总体骨密度、最大载荷、最大应力、最大能量、刚度、血清Ca水平、Col Ⅱ、AP-2 mRNA及蛋白表达水平均明显升高，血清BALP、P、PTH水平、PPAR-γ、AP-2 mRNA及蛋白表达水平均明显降低（P＜0.05）。结论维生素D可有效改善骨质疏松大鼠模型骨代谢相关指标，减少骨量缺损，其作用机制可能与调控Col Ⅱ、PPAR-γ、AP-2 mRNA及蛋白的表达有关。

英文摘要:

Objective To investigate the effect of vitamin D on the repair of bone mass loss in osteoporosis rat model by regulating the mRNA and protein expressions of type Ⅱ collagen (Col Ⅱ), peroxisome proliferator activated receptor γ (PPAR-γ), and activated protein 2 (AP-2). Methods SD male rats were randomly divided into sham operation group, osteoporosis group, and vitamin D group. Bone mineral density (BMD), femur biomechanics, and bone metabolism of rats in each group were detected. RT-PCR and Western blotting were used to detect the mRNA and protein expression levels of Col Ⅱ, PPAR-γ and AP-2 in bone tissue of rats. Results Compared to those in sham operation group, cancerous BMD, cortical BMD, total BMD, maximum load, maximum stress, maximum energy, stiffness, serum calcium (Ca) level, and Col II mRNA and protein expression levels decreased significantly in osteoporosis group. Serum bone specific alkaline phosphatase (BALP), phosphorus (P), parathyroid hormone (PTH) levels, and PPAR-γ, AP-2 mRNA and protein expression levels increased significantly (P<0.05). Compared to those in osteoporosis group, femur cancellous BMD, cortical BMD, total BMD, maximum load, maximum stress, maximum energy, stiffness, serum Ca level, and Col Ⅱ, AP-2 mRNA and protein expression levels increased significantly in vitamin D group. Serum levels of BALP, P, PTH, PPAR-γ, and AP-2 mRNA and proteins decreased significantly (P<0.05). Conclusion Vitamin D effectively improves bone metabolism and reduces bone mass loss in osteoporosis rats. Its mechanism may be related to the regulation of Col Ⅱ, PPAR-γ, and AP-2 mRNA and protein expressions.

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