|
| 骨碎补总黄酮经破骨前体/成骨细胞双向调控H型血管促进诱导膜技术骨重建 |
| Total flavonoids of rhizoma drynariae (TFRD) promotes bone reconstruction in masquelet technique by bidirectional regulation of type H vessels through osteoclast precursors and osteoblasts |
| |
| DOI：10.3969/j.issn.1006-7108.2025.12.002 |
| 中文关键词: 骨碎补总黄酮 H型血管 诱导膜 骨缺损 骨重建 |
| 英文关键词:total flavonoids of rhizomadrynariae H-type vessels induced membrane bone defect bone reconstruction |
| 基金项目:国家自然科学基金（82460938，81960880） |
|
| 摘要点击次数: 2 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的 探讨骨碎补总黄酮通过破骨前体/成骨细胞“双向”调控H血管偶联成骨促进诱导膜技术二期骨重建的分子机制。方法 将大鼠按体重分层后随机分为每组12只的空白组、模型组和骨醉补总黄酮组（TFRD组）。空白组大鼠制作4mm的股骨骨缺损模型，而模型组和TFRD组建立与之相同尺寸的Masquelet诱导膜骨缺损模型。一期造模6周后，模型组和TFRD组将大鼠尾骨颗粒植入诱导膜内，空白组植入到骨缺损处，TFRD组予157.5 mg/(kg?d)的强骨胶囊灌胃，模型组和空白组予等量生理盐水灌胃。二期植骨术后8周，使用X线和HE染色观察骨缺损区的骨重建情况和新生骨组织的形态学结构。通过免疫荧光双染色法观察新生骨组织中H型血管数量，免疫组化和Western-blot法检测破骨前体/成骨细胞相关细胞因子的表达情况。结果 X线及HE染色显示，TFRD组截骨区骨重建水平较模型组和空白组更佳，局部有更多的新生骨组织（P＜0.05）；免疫荧光双染色法显示TFRD组H型血管数量较其余两组增多（P＜0.05）；免疫组化和Western-blot显示骨碎补总黄酮可上调新生骨组织中破骨前体/成骨细胞相关细胞因子的表达（P＜0.05）。结论 骨碎补总黄酮可通过破骨前体/成骨细胞“双向”调控H型血管偶联成骨，促进诱导膜技术骨重建。 |
| 英文摘要: |
| Objective To investigate the molecular mechanism by which Total flavonoids of Rhizoma fortune promote bone reconstruction using the induced membrane technique through the "bidirectional" regulation of osteoclast precursor/osteoblasts coupling with H-type vessels. Methods Rats were stratified by weight and then randomly divided into three groups of 12 rats each: a blank group, a model group, and a traditional Chinese medicine group.The 4 mm femoral bone defect model was created in the control group, while the model and TCM groups established ainduced membrane model of equivalent size. Six weeks after the first-stage modeling, tail bone particles were implanted into the induced membrane. The TCM group was administered with a strong bone capsule at a dose of 157.5mg/kg/day by gavage, while the model and control groups received an equal volume of normal saline. Eight weeks after the second-stage bone grafting, X-ray and HE staining were used to observe bone reconstruction and the morphological structure of new bone tissue in the defect area. H-type vessel count in the new bone tissue was observed by immunofluorescent double staining, and the expression of H-type vessel-related factors was detected by immunohistochemistry and Western blot. Results X-ray and HE staining showed improved bone regeneration in the TCM group versus the model and control groups, featuring increased new bone and vascular tissue within the defect site (P<0.05).Immunofluorescent double staining showed a significant increase in the number of H-type vessels in the TCM group (P<0.05). Immunohistochemistry and Western blot indicated that Total flavonoids of RhizomaDrynariae significantly upregulated the positive expression of H-type vessels in the new bone tissue (P<0.05). Conclusion The total flavonoids of rhizomadrynariae can regulate H-type vascular coupling osteogenesis through a "bidirectional" approach of osteoclasts/osteoblasts, promoting induced membrane technology for bone reconstruction. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |