|
| 羊胎盘肽改善卵巢切除大鼠骨质疏松作用研究 |
| Research on the effect of sheep placental peptide in improving osteoporosis in ovariectomized rats |
| |
| DOI：10.3969/j.issn.1006-7108.2026.02.005 |
| 中文关键词: 羊胎盘肽 绝经后骨质疏松 骨密度 Micro-CT 骨生物力学 骨代谢 |
| 英文关键词:sheep placental peptide postmenopausal osteoporosis bone mineral density micro-CT bone biomechanics bone metabolism |
| 基金项目:甘肃省科技重大专项（24ZDFA008）；甘肃省自然科学基金（22JR11RA013）；兰州市科技计划项目（2023-1-8，2023-ZD-183） |
| 作者 | 单位 | | 王立强1,2,3 安梓栋1,2,3 庞永杰2,3 武艺1,3 杨世超1,2,3
宋慕格1,2,3 高玉海2,3 陈克明1,2,3* | 1.甘肃中医药大学中医临床学院,甘肃 兰州 730000
2.中国人民解放军联勤保障部队第九四〇医院基础医学实验室,甘肃 兰州 730050
3.甘肃省干细胞与基因药物重点实验室,甘肃 兰州 730050 |
|
| 摘要点击次数: 6 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的 探究羊胎盘肽预防去卵巢大鼠发生骨质疏松的作用。方法 取3月龄雌性SD大鼠60只，随机分为6组：假手术组（Sham)、模型组（OVX）、羊胎盘肽低（L）、中（M）、高（H）剂量组、仙灵骨葆组（XLGB）。除Sham组去除等体积脂肪外，均建立卵巢切除模型。羊胎盘肽组低、中、高剂量分别为150、300、600 mg/（kg·d），XLGB组给予仙灵骨葆胶囊315 mg/（kg·d），Sham组与OVX组给予等体积蒸馏水，1次/d，连续灌胃给药8周，每周记录体质量。8周后取材，计算脏器系数，行股骨与椎骨骨密度检测、Mirco-CT扫描、生物力学试验、骨组织形态及血清骨代谢生化指标检测等。结果 主要脏器中Sham组子宫系数显著高于OVX组，肝、心、脾、肺、肾等脏器未发现明显改变，脏器系数差异无统计学意义（P＞0.05）；L组股骨离体骨密度明显高于OVX组（P＜0.05）；Micro-CT结果显示，L组骨矿物质密度（Tb.BMD）、骨小梁数量（Tb.N）、骨体积分数（Tb.BV/TV）与OVX组相比，均有明显增加，但骨小梁分离度（Tb.Sp）数值减少显著（P＜0.05）；骨生物力学试验结果显示，与OVX组相比，L组最大载荷和弯曲强度均显著升高（P＜0.05）；股骨HE染色显示羊胎盘肽组骨小梁形态与结构更接近Sham组；L组血清中骨形成指标BALP、PINP与OVX组相比明显上升，骨吸收指标TRAP、CTX-1明显下降（P＜0.05）。结论 羊胎盘肽通过促进骨形成、抑制骨吸收的双重机制，有效改善卵巢切除诱导的骨丢失，其中低剂量[150 mg/（kg·d）]对骨质疏松的预防效果最佳。其作用可能与调节成骨细胞活性、抑制破骨细胞功能及改善骨微环境相关。 |
| 英文摘要: |
| Objective To investigate the preventive effects of sheep placental peptide (SPP) on osteoporosis in ovariectomized (OVX) rats. Methods Sixty 3-month-old female Sprague-Dawley (SD) rats were randomly allocated into six groups: sham-operated (Sham), OVX model (OVX), low-dose SPP (L, 150 mg/kg·d), medium-dose SPP (M, 300 mg/kg·d), high-dose SPP (H, 600 mg/kg·d), and Xianling Gubao (XLGB: 315 mg/kg·d) group. Bilateral ovariectomy was performed in all groups except the Sham group, which underwent equivalent adipose tissue removal. Daily intragastric administration of SPP, XLGB capsules, or distilled water (Sham and OVX groups) was conducted for 8 weeks, with weekly body weight monitoring. Post-intervention evaluations included organ coefficients, bone mineral density (BMD) of the femoral and vertebral bone, micro-CT analysis, biomechanical testing, bone histomorphology, and serum bone metabolism biomarkers. Results The uterine coefficient in the Sham group was significantly higher than that in the OVX group (P<0.05), while no significant differences were observed in the liver, heart, spleen, lung, or kidney coefficients (P>0.05). The L group exhibited significantly higher femoral BMD than the OVX group (P<0.05). Micro-CT analysis revealed that the L group showed increased trabecular BMD (Tb.BMD), trabecular number (Tb.N), and bone volume fraction (Tb.BV/TV), but reduced trabecular separation (Tb.Sp) compared to the OVX group (P<0.05). Biomechanical testing demonstrated significant improvements in maximum load and bending strength in the L group versus OVX (P<0.05). Hematoxylin-eosin (HE) staining of the femurs indicated that the trabecular morphology and structure in SPP-treated groups closely resembled the Sham group. Serum analysis revealed elevated bone formation markers (BALP and PINP) and reduced bone resorption markers (TRAP and CTX-1) in the L group compared to OVX (P<0.05). Conclusion SPP effectively ameliorates ovariectomy-induced bone loss through dual mechanisms of promoting osteogenesis and suppressing bone resorption, with the low-dose regimen (150 mg/kg·d) demonstrating optimal efficacy in osteoporosis prevention. These effects may be attributed to modulation of osteoblast activity, inhibition of osteoclast function, and improvement of the bone microenvironment. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|