中国骨质疏松杂志

异荭草素调节NF-κB/MAPK通路对骨质疏松老年大鼠的治疗作用

Therapeutic effect of isoorientin on elderly rats with osteoporosis by regulating NF-κ B / MAPK pathway

DOI：10.3969/j.issn.1006-7108.2026.06.009

英文关键词:isoorientin NF-κB/MAPK signaling pathway osteoporosis elderly

基金项目:长沙市自然科学基金（kq2208460）

作者	单位
王聪钟丽颖*	长沙市第三医院老年病科,湖南长沙 410015

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中文摘要:

目的探讨异荭草素（Iso）调节NF-κB/MAPK信号通路对骨质疏松老年大鼠的治疗作用。方法将构建的骨质疏松老年大鼠随机分为模型组、Iso低剂量（50 mg/kg）组、Iso高剂量（100 mg/kg）组、阿仑膦酸钠（1.428 mg/kg）组、Iso高剂量+激活剂（100 mg/kg Iso+5 mg/kg佛波酯）组；并以暴露子宫，仅切除卵巢周围脂肪的大鼠为假手术组，治疗结束后，检测骨密度值及骨小梁分离度（Tb.SP）、小梁厚度（Tb.Th）以及骨小梁数量（Tb.n）；分离血清，检测骨碱性磷酸酶（BAP）、I型前胶原羧基末端前肽（PICP）、骨钙素（OCN）及白细胞介素（IL）-6、IL-1β、破骨细胞特异性酶（TRACP 5b）和I型胶原降解产物（CTX-Ⅰ）水平；分离股骨组织，检测病理学变化及NF-κBp65、p38磷酸化表达。结果模型组骨密度、BAP、OCN、Tb.SP、Tb.Th、Tb.n较假手术组降低，PICP、IL-6、IL-1β、TRACP 5b、CTX-Ⅰ、p-NF-κBp65/NF-κBp65、p-p38/p38、p-ERK/ERK表达增加（P<0.05）；不同剂量Iso组、阿仑膦酸钠组骨密度、BAP、OCN、Tb.SP、Tb.Th、Tb.n较模型组增加，PICP、IL-6、IL-1β、TRACP 5b、CTX-Ⅰ、p-NF-κBp65/NF-κBp65、p-p38/p38、p-ERK/ERK表达降低，不同剂量Iso差异具有统计学意义（P<0.05）；Iso高剂量+激活剂组骨密度、BAP、OCN、Tb.SP、Tb.Th、Tb.n较Iso高剂量组降低，PICP、IL-6、IL-1β、TRACP 5b、CTX-Ⅰ、p-NF-κBp65/NF-κBp65、p-p38/p38、p-ERK/ERK表达增加（P<0.05）。结论 Iso通过调节NF-κB/MAPK信号通路减轻骨质疏松老年大鼠病理损伤，发挥抗骨质疏松作用。

英文摘要:

Objective To investigate the therapeutic effect of isoorientin (Iso) on elderly rats with osteoporosis by regulating the NF-κB/MAPK signaling pathway. Methods The constructed elderly rats with osteoporosis were randomly divided into model group, Iso low-dose (50 mg/kg) group, Iso high-dose (100 mg/kg) group, alendronate sodium (1.428 mg/kg) group, Iso high-dose+activator (100 mg/kg Iso+5 mg/kg phorbol ester) group, and the rats with exposed uteri and only the fat around the ovaries removed were taken as the sham operation group. After treatment, the bone mineral density values, trabecular bone separation degree (Tb.SP), trabecular bone thickness (Tb.Th), and trabecular bone quantity (Tb.n) were detected. Serum was isolated and the levels of Bone alkaline phosphatase (BAP), carboxyl-terminal propeptide of type I procollagen (PICP), osteocalcin (OCN), interleukin (IL) -6, IL-1β and osteoclast-specific enzyme (TRACP 5b) and type I collagen degradation products (CTX-I) were detected. The femoral tissues were isolated to detect the pathological changes and the phosphorylated expressions of NF-κBp65 and p38. Results The bone mineral density, BAP, OCN, Tb.SP, Tb.Th and Tb.n in the model group were lower than those in the sham operation group, while the expressions of PICP, IL-6, IL-1β, TRACP 5b, CTX-I, p-NF-κBp65/NF-κBp65, p-p38/p38 and p-ERK/ERK increased (P<0.05). Bone mineral density, BAP, OCN, Tb.SP, Tb.Th and Tb.n in different dose Iso groups and alendronate sodium groups increased compared with the model group, while the expressions of PICP, IL-6, IL-1β, TRACP 5b, CTX-I， p-NF-κBp65/NF-κBp65, p-p38/p38 and p-ERK/ERK decreased. There were statistically significant differences in Iso doses (P<0.05). Compared with the high-dose Iso group, the pathological damage in the Iso high-dose+activator group was aggravated, the bone density, BAP, OCN, Tb.SP, Tb.Th and Tb.n were obviously decreased, the expression levels of PICP, IL-6, IL-1β, TRACP 5b, CTX-I， p-NF Bp65/NF Bp65, p-p38/p38, and p-ERK/ERK were obviously increased (P<0.05). Conclusion Iso reduces pathological damage and exerts anti osteoporosis effects in elderly rats with osteoporosis by regulating the NF-κB/MAPK signaling pathway.

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