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| GSK-1520489A对骨髓间充质干细胞影响实验研究 |
| Experimental study of the effect of GSK-1520489A on bone marrow mesenchymal stem cells |
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| DOI：10.3969/j.issn.1006-7108.2026.06.010 |
| 中文关键词: GSK-1520489A 人骨髓间充质干细胞 成骨分化 |
| 英文关键词:GSK-1520489A human bone marrow mesenchymal stem cells osteogenic differentiation |
| 基金项目:国家自然科学基金（82260429；82260434）；贵州医科大学附属医院学科优秀后备人才项目（gyfyxkrc-2023-07）；贵州医科大学附属医院国家自然科学基金（NSFC）面上基金培育计划项目[gyfynsfc（2023）-02] |
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| 中文摘要: |
| 目的 观察和比较不同浓度的GSK-1520489A对人骨髓间充质干细胞(bone marrow-derived mesenchymal stem cells，BMSCs)成骨分化的影响。方法 从人骨髓中分离培养出骨髓间充质干细胞，分别用0、50、100、150、200、250、300、350、400 nmol/mL浓度的GSK-1520489A处理BMSCs，运用细胞计数试剂盒（CCK8）法检测GSK-1520489A对骨髓间充质干细胞的活性影响；蛋白质印迹法（Western blot）检测经GSK-1520489A处理的人骨髓间充质干细胞中成骨标志物蛋白碱性磷酸酶（ALP）和骨桥蛋白（osteopontin， OPN）的表达情况；碱性磷酸酶（alkaline phosphatase， ALP）和茜素红S（alizarin red， ARS）染色鉴定GSK-1520489A对BMSCs的成骨分化。通过Western blot检测 GSK-1520489A促进骨髓间充质干细胞成骨分化的机制。结果 当GSK-1520489A浓度为200 nmol/mL时, BMSCs的活性[（87.207±4.214）%]与对照组（0 nmol/mL）相比， 无统计学差异（P＞0.05），GSK-1520489A浓度在0~200 nmol/mL范围时，Western Blot显示成骨因子ALP和矿化因子OPN的表达随着GSK-1520489A浓度的增加逐渐增强。ALP和ARS染色结果显示，GSK-1520489A促进BMSCs成骨分化。结论 GSK-1520489A通过?Wnt/β-catenin通路促进了BMSCs的成骨分化，且浓度在0~200 nmol/mL内，分化能力随着浓度的增加逐渐增强。 |
| 英文摘要: |
| Objective To observe and compare the effects of different concentrations of GSK-1520489A on osteogenic differentiation of human bone marrow derived mesenchymal stem cells (BMSCs). Methods Bone marrow mesenchymal stem cells were isolated and cultured from human bone marrow. hBMSCs were treated with GSK-1520489A at concentrations of 0, 50, 100, 150, 200, 250, 300, 350, and 400 nmol/mL, respectively. The activity of GSK-1520489A on bone marrow mesenchymal stem cells was detected using a cell counting kit (CCK8). Western blotting was used to detect the expressions of osteogenic marker alkaline phosphatase (ALP) and osteopontin (OPN) in human bone marrow mesenchymal stem cells treated with GSK-1520489A. The osteogenic differentiation of hBMSCs by GSK-1520489A was identified using alkaline phosphatase (ALP) and Alizarin red (ARS) staining. Western blotting was used to detect the mechanism of GSK-1520489A promoting osteogenic differentiation of bone marrow mesenchymal stem cells. Results The activity of hBMSCs (87.207 ± 4.214)% was not significantly different compared to the control at 200 nmol/mL of GSK-1520489A (0 nmol/mL, P>0.05). Within the range of 0-200 nmol/mL, Western blotting results showed that the expressions of osteogenic factor ALP and mineralization factor OPN gradually increased with the increase of GSK-1520489A concentration. The ALP and ARS staining results showed that GSK-1520489A promoted osteogenic differentiation of hBMSCs. Conclusion GSK-1520489A promotes osteogenic differentiation of hBMSCs through the Wnt/β - catenin pathway. The osteogenic differentiation ability of bone marrow mesenchymal stem cells gradually increases with increasing concentration within 0-200 nmol/mL of GSK-1520489A. |
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