骨形态计量学目前应用专家共识
Expert consensus about the current application of bone histomorphometry
  
DOI:
中文关键词:  骨组织形态计量学  标准命名法  骨代谢  骨矿化
英文关键词:Bone histomorphometry  Standard nomenclature  Bone metabolism  Bone mineralization
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作者单位
陈珺;张豪;杨国柱;陆幸研;金小冬;卢丽;李青南 广东药学院生命科学与生物制药学院骨代谢研究室 
摘要点击次数: 2004
全文下载次数: 2891
中文摘要:
      1960年,美国骨整形外科医生Harold Frost提出一种定量研究骨微结构和骨量的技术一骨组织形态计量学(BHMT), 其采用不脱钙硬包埋法和活体荧光标记法进行骨组织标本制作,更好地保留了骨组织微细结构。50多年来的实践证实 BHMT是目前评价骨转换与骨矿化最常用和有效的实验手段,至今仍是骨代谢和矿化评价的金标准。目前国际上有两套常用 的标准骨组织形态计量学方法:一是Harold Frost与美国犹他大学医学院放射生物系Webster S. S. Jee教授及Sun Valley Hard Tissue Workshops 一起发展并提出的MMT标准命名法,下文简称Dr. Frost and Jee教授团队命名法。二是在美国医生A. Michael Parfitt于1980年提出的一套强调骨组织微结构研究重要性的体视学测量方法基础上,由美国骨矿研究协会(ASBMR) 综合提出的标准命名法(1987年第一版和2012年第二版)。上述两种系统命名法均使用广泛,每套系统的指标都很多且各有 侧重,使用时可按具体实验需要选择最为适用的系统。本文将讨论2种方法的异同点及其在BHMT研究中的应用。
英文摘要:
      As a quantitative research technique of bone microstructure and bone mass,bone histomorphometry (BHMT) has been proposed by Harold Frost,an American orthopedic surgeon,in 1960. For better preservation of bone microstructure,undecalcified embedding and fluorescent labeling in vivo are used to make bone tissue samples for BHMT. More than fifty-year application has proved that BHMT is not only the most common and effective method used for the evaluation of bone turnover and bone mineralization,but also the golden standard for the evaluation of bone metabolism and mineralization. There are two common bone histomorphometry methods. One is proposed by Harold Frost and Webster S. S. Jee,a professor from the Department of Radiation Biology,the Medical College of Utah University,and Sun Valley Hard Tissue Workshops (abbreviated as BHMT nomenclature system). Another method is developed by the American Society for Bone and Mineral Research (ASBMR),based on a set of stereological techniques for bone microarchitecture measurement,which was created by Dr. Parfitt in 1980. Both nomenclature systems have their own parameters and focus,and are widely used. It needs to choose the most appropriate system according to the particular circumstances. In this paper,we discuss the similarities and differences between the two bone histomorphometry methods, and introduce their main application.
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