OBJECTIVE:To screen the target and signaling pathway of effective ingredients of Rhizoma Drynariae in the treatment of osteoporosis by network pharmacology. METHODS:Firstly, TCMSP analysis platform , GeneCards database and OMIM database were used to screen the active ingredients and corresponding targets of Traditional Chinese Medicine(TCM), and the common target of TCM-Disease was obtained by intersecting the target of TCM with the target of disease, and the regulatory network of TCM-Disease-Target was constructed. Then, the core target genes were screened by constructing the protein protein interaction(PPI)network of common target of TCM-Disease. Finally, GO function enrichment analysis and KEGG function enrichment analysis were carried out on the common target of TCM-Disease, and the signal pathways related to the common target of TCM-Disease were obtained. RESULTS:18 effective ingredients and 98 common targets of TCM-Disease were screened for the treatment of osteoporosis. The mechanism of action may be related to AGE-RAGE signaling pathway, TNF signaling pathway, IL-17 signaling pathway, HIF-1 signaling pathway, Th17 cell differentiation signaling pathway, and interleukin-6 gene, JUN gene, mitogen-activated protein kinase-1(MAPK-1) gene and epidermal growth factor(EGF)gene may play a key role. CONCLUSION:Based on network pharmacology, the target and signal pathway of Rhizoma Drynariae in the treatment of osteoporosis are discussed, which provides ideas for the modernization of TCM. |