基于网络药理学探讨骨碎补治疗骨质疏松症的作用机制
Research on the Mechanism of Rhizoma Drynariae in the Treatment of Osteoporosis Based on Network Pharmacology
投稿时间:2019-04-18  修订日期:2019-05-29
DOI:
中文关键词:  网络药理学  骨碎补  骨质疏松症  信号通路
英文关键词:network pharmacology  rhizoma drynariae  osteoporosis  signaling pathway
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
作者单位邮编
马江涛 广州中医药大学 510405
万雷 广州中医药大学第三附属医院 
黄宏兴* 广州中医药大学第三附属医院 510375
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中文摘要:
      目的 运用网络药理学筛选骨碎补有效成分治疗骨质疏松症的作用靶标及信号通路。方法 首先借助TCMSP分析平台、GeneCards数据库和OMIM数据库,分别筛选中药活性成分、疾病及相应的靶标,将中药靶标与疾病靶标取交集,得到中药-疾病共同靶标并构建中药-疾病-靶标调控网络。然后通过构建中药-疾病共同靶标的蛋白互作网络,筛选核心靶点基因。最后将中药-疾病共同靶标进行GO功能富集分析和KEGG功能富集分析,得出中药-疾病共同靶标相关的信号通路。结果 筛选得到骨碎补治疗骨质疏松症的18个有效成分和98个中药-疾病共同靶标,并推断其作用机制可能与糖尿病并发症的AGE-RAGE信号通路、TNF信号通路、IL-17信号通路、HIF-1信号通路、Th17细胞分化信号通路等有关,且白介素-6基因、JUN基因、丝裂原活化蛋白激酶-1基因和表皮细胞生长因子基因可能起着关键性作用。结论 基于网络药理学探讨了骨碎补治疗骨质疏松症的作用靶标及信号通路,为中药现代化研究提供了思路。
英文摘要:
      OBJECTIVE:To screen the target and signaling pathway of effective ingredients of Rhizoma Drynariae in the treatment of osteoporosis by network pharmacology. METHODS:Firstly, TCMSP analysis platform , GeneCards database and OMIM database were used to screen the active ingredients and corresponding targets of Traditional Chinese Medicine(TCM), and the common target of TCM-Disease was obtained by intersecting the target of TCM with the target of disease, and the regulatory network of TCM-Disease-Target was constructed. Then, the core target genes were screened by constructing the protein protein interaction(PPI)network of common target of TCM-Disease. Finally, GO function enrichment analysis and KEGG function enrichment analysis were carried out on the common target of TCM-Disease, and the signal pathways related to the common target of TCM-Disease were obtained. RESULTS:18 effective ingredients and 98 common targets of TCM-Disease were screened for the treatment of osteoporosis. The mechanism of action may be related to AGE-RAGE signaling pathway, TNF signaling pathway, IL-17 signaling pathway, HIF-1 signaling pathway, Th17 cell differentiation signaling pathway, and interleukin-6 gene, JUN gene, mitogen-activated protein kinase-1(MAPK-1) gene and epidermal growth factor(EGF)gene may play a key role. CONCLUSION:Based on network pharmacology, the target and signal pathway of Rhizoma Drynariae in the treatment of osteoporosis are discussed, which provides ideas for the modernization of TCM.
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