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| 补肾健脾中药复方对去势大鼠骨骼肌线粒体通透转孔调控的研究 |
| Study of Chinese herbal compound for tonifying the kidney and invigorating the spleen on the modulation of skeletal muscle mitochondrial permeability and transition pore in ovariectomized rats |
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| DOI：10. 3969/j. issn. 1006-7108. 2012. 12. 016 |
| 中文关键词: 去势大鼠 中药复方 线粒体通透转换孔 骨骼肌 |
| 英文关键词:Ovariectomized rats Chinese herbal compound Mitochondrial permeability transition pore Skeletal muscle |
| 基金项目:国家自然科学基金（81001532 )中国博士后基金（20100480737 );广东省科技计划项目 (2010B030700076 );广州市中医药科技项目（201122231001 ) |
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| 中文摘要: |
| 目的 探讨补肾健脾中药复方干预去势大鼠骨骼肌线粒体通透转孔的影响。方法 48只6 月龄的雌性SD大鼠随机分为空白组、模型组、中药组、西药组。治疗12周后收集左侧股骨、股直肌分 别行骨矿含量、骨密度测量，同时进行骨骼肌线粒体通透转换孔的检测。结果 最后纳入研究的动物 40只。模型组的骨密度值和骨矿含量均低于空白组，其差异有统计学意义（P <0.05 );中药组和西药组的骨密度值均高于模型组，其差异有统计学意义（P <0.05 )。在骨骼肌线粒体通透转换孔方面，空白组的OD比值均相应高于模型组的OD比值，其差异有统计学意义（P <0.05 )。经过药物干预后， 观察组的OD比值与对照组的OD比值均相应低于同一时间点的模型组OD比值，其差异有统计学意 义（P <0.05 )。结论 补肾健脾中药复方对去势大鼠有明显的治疗作用，能明显提高骨密度；而且能在一定程度抑制骨骼肌细胞的凋亡。 |
| 英文摘要: |
| Objective To study the effect of the Chinese herbal compound for tonifying the kidney and invigorating the spleen on skeletal muscle mitochondrial permeability transition pore ( MPTP ) in ovariectomized rats. Methods Forty-eight 6-month-old female SD rats were randomly divided into 4 groups: control group, model group, traditional Chinese herb group， and western medicine group. After a 12-week treatment, the left femur and the left rectus femoris were collected. Bone mineral density ( BMD ) and bone mineral content(BMC ) were measured using dual energy X-ray absorptiometry. MPTP was detected with colorimetry. Results Forty rats were involved in the result analysis eventually. BMD and BMC in model group were lower than those in control group, and the difference was significant ( P < 0. 05 ). BMD of both traditional herb group and western medicine group was higher than that in model group, and the difference was significant ( P <0.05 ). As to MPTP，the OD ratio in control group was higher than that in model group, and the difference was significant ( P < 0. 05 ). After the drug intervention，the OD ratio in control group， traditional Chinese herb group，and western medicine group was lower than that in model group at the same time point，and the difference was significant( P <0. 05 ). Conclusion The Chinese herbal compound for tonifying the kidney and invigorating the spleen has an obvious treatment effect on ovariectomized rats. It can improve BMD significantly and inhibit the apoptosis of skeletal muscle cells to some extent. |
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