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| 瑞舒伐他汀对体外培养人成骨细胞增殖及LRPS、Runx2基因表达的影响 |
| Effect of rosuvastatin on the proliferation of human osteoblasts in vitro and the gene expression of LRPS and Runx2 |
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| DOI：10.3969/j.issn.1006.7108.2013.11.003 |
| 中文关键词: 瑞舒伐他汀 成骨细胞 增殖 LRPS Runx2 |
| 英文关键词:Rosuvastatin Osteoblast Proliferation LRPS Runx2 |
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| 中文摘要: |
| 目的 观察不同浓度的瑞舒伐他汀对体外培养的人成骨细胞增殖及LRPS,Runx2基因表达的影响，探讨瑞舒伐他汀影响骨代谢可能的分子机制。方法 (1)成骨细胞的培养与鉴定;(2)给予不同浓度(0,10-6 , 10-7 ,10-8,10-9 mol/L)的瑞舒伐他汀刺激体外培养的人成骨细胞,24 h后采用CCK-8比色法检测细胞的增殖能力，用荧光定量PCR法检测LRPS , Runx2的相对表达水平。结果 瑞舒伐他汀可明显促进细胞增殖(P<0.05)，并促进成骨细胞LRPS , Runx2基因的表达(P<0.05),10-7mol/L增殖最明显、基因的表达量最高。结论 瑞舒伐他汀可促进成骨细胞的增殖，可能与LRPS , Runx2基因表达增加有关。 |
| 英文摘要: |
| Objective To observe the effect of different concentrations of rosuvastatin on the proliferation of human osteoblasts in vitro and the gene expression of LRPS and Runx2, and to investigate the possible mechanism of rosuvastatin in bone metabolism.Methods First，osteoblasts were cultured in vitro and identified .Different concentrations(0，10-6，10-7，10-8，and 10-9 mol/L)of rosuvastatin were additioned to stimulate the human osteoblasts in vitro .After 24 hours，cell proliferation was detected using CCK-8 colorimetnc method. The relative expression of LRPS and Runx2 was detected using fluorescencx quantitative PCR assay .Results Rosuvastatin significantly promoted cell proliferation(P<0.05)，and increased the gene expression of LRPS and Runx2(P<0.05). The concentration of 10-7 mol/L showed the most obvious effect in proliferation and had the highest amount of gene expression .Conclusion Rosuvastin can promote the proliferation of osteoblasts，which may he associate with increased expression of LRP 5 and Runx2. |
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