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| 电针对去卵巢大鼠骨形成及骨吸收活性的影响 |
| Effect of electroacupuncture on the bone formation and bone resorption in ovariectomized rats |
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| DOI：10.3969/j.issn.1006-7108.2017.05.006 |
| 中文关键词: 电针 骨质疏松症 BMD BMC OPG RANKL |
| 英文关键词:Electroacupuncture Osteoporosis BMD BMC OPG RANKL |
| 基金项目:国家自然科学基金地区基金项目（81260584） |
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| 中文摘要: |
| 目的 研究电针刺对去卵巢骨质疏松模型大鼠的全身骨密度（BMD）、骨矿物含量（BMC）及其血清对体外培养成骨细胞的骨形成因子骨钙素（OPG）和骨吸收因子（RANKL）蛋白表达的影响。方法 选取SPF级2月龄SD雌性大鼠40只，随机分为空白组、电针组、药物组和模型组，每组10只。其中空白组不做任何处理，后3组采用手术方法切除双侧卵巢。饲养3个月后，骨密度检测结果显示造模成功。随后药物组大鼠灌胃服用戊酸雌二醇3个疗程，电针组大鼠采用针刺后通电治疗3个疗程并服用药物组相同体积的蒸馏水，模型组服用相应体积的蒸馏水，空白组常规饲养。各组大鼠于SPF级实验室处理3个月后，双能X射线骨密度仪检测全身骨密度和骨矿含量。随后，水合氯醛麻醉各组大鼠，心脏采血,各组血液分别经滤网过滤、56℃水浴处理后加入体外培养的大鼠成骨细胞培养液中，处理3d后多聚甲醛固定成骨细胞，采用免疫细胞化学染色法检测成骨细胞内OPG、RANKL蛋白的表达量。结果 与空白组相比，模型组大鼠BMD,BMC显著降低（P<0.05），服用药物或针刺处理后，BMD和BMC显著增高(P<0.05)，与模型组相比差异具有统计学意义。各组大鼠血清处理成骨细胞后，模型组骨形成指标OPG蛋白表达量较低，经药物或针刺后，OPG蛋白表达量显著升高（P<0.05）。此外，模型组骨吸收指标RANKL的表达量较高，经药物和针刺处理后，RANKL蛋白表达量显著下降（P<0.05）。结论 针刺能有效升高大鼠BMD和BMC，同时针刺处理后的大鼠血清具有很好的促进骨形成和抑制骨吸收的能力。 |
| 英文摘要: |
| Objective To observe the effect of electroacupuncture on bone mineral density (BMD) and bone mineral content (BMC) in ovariectomized rats, and on the expression of OPG and RANKL in cultured osteoblasts in vitro. Methods Forty 2-month-old female SPF-grade SD rats were selected and were randomly divided to blank group, electroacupuncture group, Western medicine group, and model group, with 10 rats in each group. Rats were bilaterally ovariectomized except in the blank group. The model was established in 3 months according to the BMD results. Rats in the western group were then administrated with estradiol valerate by means of intragastrical administration for 3 course. Rats in the electroacupuncture group were treated with acupuncture and intragastrical administration of same volume of distilled water for 3 month. Rats in the model group were administrated with distilled water for 3 month. Rats in the blank group were fed normally. After 3 months in the SPF laboratory, BMD and BMC were detected using dual energy X-ray absorptiometry. The rats of all the groups were anesthetized with chloral hydrate and blood were collected from the heart. The blood was mesh filtered and dealt with 56?C water bath. Then it was added in the cultured osteoblasts. The osteoblasts were fixed with paraformaldehyde after 3 days. The protein expression of OPG and RANKL in the osteoblasts were detected using immunofluorescence cytochemistry. Results Comparing to those in the blank group, BMD and BMC in the model groups decreased significantly (P<0.05). After the medicine or acupuncture treatment, BMD and BMC increased significantly, comparing to those in the model group (P<0.05). The protein expression of OPG was low in the osteoblasts cultured with the model group serum. It increased after the medicine or acupuncture treatment (P<0.05). The expression of RANKL was the highest in the model group. After the treatment, it decreased in the western group and the electroacupuncture group (P<0.05). Conclusion The treatment of electroacupuncture increases BMD and BMC in rats. After the treatment with electroacupuncture, the rat serum has good capacity of increasing bone formation and decreasing bone resorption. |
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