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| 补肾健脾活血方干预过表达DKK1骨细胞对细胞活性及BMP2的影响 |
| The effects of bushen jianpi huoxue recipe on cell activity and BMP2 expression in DKK1 over-expressed osteoblasts |
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| DOI：10.3969/j.issn.1006.7108.2017.06.003 |
| 中文关键词: 中医中药 骨质疏松症 DKK1 BMP2 补肾健脾活血方 |
| 英文关键词:Traditional Chinese medicine Osteoporosis DKK1 BMP2 Bushen jianpi huoxue recipe |
| 基金项目:国家自然科学基金课题（81302991);国家自然科学基金课题(81674004);广东省自然科学基金课题(S2013040016828) |
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| 中文摘要: |
| 目的 用补肾健脾活血方干预过表达的DKK1腺病毒转染的成骨细胞，通过观察细胞的活性及BMP2的表达，研究补肾健脾活血方治疗骨质疏松的分子生物学机制。方法 将培养细胞分成NC对照组，Ad-DKKl组两组。NC对照组由空载腺病毒转染；Ad-DKK-1组由包装过表达的DKK1腺病毒转染。每组分别用含药血清和空白血清干预。采用CKK-8检测细胞增殖，蛋白印记法检测BMP2的表达。结果 各组细胞活性变化:空白血清和含药血清干预均能提高细胞活性。与空白血清分别干预的两组比较,含药血清分别干预后的两组，在24 h,48 h,72 h的细胞活性的增加倍数都较多；在采用蛋白印记法检测后，BMP2在含药血清分别干预的两组的相对表达量较空白血清分别干预的两组的相对表达量髙（P <0.01，P<0.01),在NC对照组中含药血清干预与空白血清干预无明显差异。结论 补肾健脾活血方含药血清可以提高细胞活性，提高BMP2的相对表达量，有利于成骨细胞的矿化,尤其在过表达DKK1干预后。补肾健脾活血方可能通过调控DKKI进而影响成骨细胞的代谢。 |
| 英文摘要: |
| Objective To study the molecular biological mechanism of Bushen Jianpi Huoxue Recipe in the treatment of osteoporosis by observing its effects on cell activity and BMP2 expression in DKK1 over-expressed osteoblasts. Methods Cultured cells were divided into two groups：NC group and Ad-DKKl group. NC control group was transfected with empty adenovirus, and Ad-DKK-l group was transfected with DKK1 adenovirus. Each group was treated with medicated serum and blank serum. CKK-8 was used to detect the proliferation of cells and the expression of BMP2 was detected by western blot. Results The changes of cell activity in each group: both blank serum and serum containing drug could improve the activity of cells. Compared with the two groups treated by blank serum，the two groups treated by medicated serum had greater multiple increase in cell activity at 24 h, 48 h and 72 h. Using western blot, compared with the two groups treated by blank serum, the two groups treated by medicated serum had relatively greater BMP2 expression (P<0. 01, P <0. 01) , but in the control group the serum and blank serum intervention had no significant difference. Conclusion The serum containing Bushen Jianpi Huoxue Recipe can improve the activity of cells and increase the relative expression of BMP2, which is beneficial to the mineralization of osteoblasts, especially in the over expression of DKK1. Bushen Jianpi Huoxue Recipe may affect the metabolism of osteoblasts through regulating DKK1. |
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