中国骨质疏松杂志

促胰液素对去卵巢骨质疏松大鼠血清骨转换指标和骨密度的影响

Effect of secretin on serum bone turnover markers and bone mineral density in ovariectomized rats

DOI：10.3969/j.issn.1006-7108.2019.05.023

英文关键词:secretin ovariectomized rat bone turnover marker bone mineral density animal experiment

基金项目:高等学校博士学科点专项科研基金项目(20133322110005)

作者	单位
张佳锋1 陈文亮1 毛一凡1 刘钟1 梁博程2 史晓林2*	1.浙江中医药大学，浙江杭州 310053 2.浙江中医药大学附属第二医院，浙江杭州 310005

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中文摘要:

目的观察促胰液素(secretin，SCT)对去卵巢骨质疏松大鼠骨转换指标和骨密度的影响。方法采用双侧卵巢去除法制备绝经后骨质疏松大鼠模型，将60只SD大鼠随机分为假手术组、模型对照组、雌激素治疗组和促胰液素治疗组，每组各15只。干预3个月后，测定腰椎骨密度(bone mineral density，BMD)，采取ELISA法测定血清I型胶原N前端肽(procollagen I N-Terminal propeptide，PINP)和I型胶原C末端肽(collagen type I C-terminal cross-linked telopeptide，CTX)，另使用STRING10.0蛋白相互作用网络分析工具分析骨质疏松相关差异蛋白。结果与假手术组比较，模型对照组、雌激素治疗组、促胰液素治疗组的PINP含量升高（P＜0.05），模型对照组的CTX含量升高（P＜0.05），模型对照组的BMD下降（P＜0.05），雌激素治疗组和促胰液素治疗组的CTX、BMD含量无显著差异（P＞0.05）；与模型对照组比较，雌激素治疗组、促胰液素治疗组PINP、CTX含量有所下降，而BMD含量升高（P＜0.05）；雌激素组与促胰液素组之间PINP、CTX、BMD含量无显著差异（P＞0.05）。结论促胰液素能改善去卵巢骨质疏松大鼠的PINP和CTX含量，增加骨密度，抑制骨质丢失，具有较好的抗骨质疏松效果。

英文摘要:

Objective To observe the effect of secretin (SCT) on bone turnover biomarker and bone mineral density (BMD) in ovariectomized rats with osteoporosis. Methods A rat model of postmenopausal osteoporosis was prepared by bilateral ovarian removal. Sixty SD rats were randomly divided into sham operation group, control group, estrogen treatment group, and SCT treatment group, with 15 rats in each group. After 3 months of treatment, BMD of the lumbar vertebrae was measured. Serum procollagen I N-Terminal propeptide (PINP) and collagen type I C-terminal cross-linked telopeptide (CTX) were determined with ELISA. In addition, osteoporosis-related proteins were analyzed using a protein interaction network analysis tool (STRING 10.0). Results Compared with the sham operation group, the PINP levels in the model control group, the estrogen treatment group, and the secretin treatment group increased (P<0.05), and the CTX levels in the control group increased (P<0.05). BMD in the control group decreased (P<0.05). There was no significant difference in the levels of CTX and BMD between the estrogen-treated group and the SCT treatment group (P>0.05). Compared with the control group, the levels of PINP and CTX in the estrogen-treated group and the secretin treatment group decreased, while BMD increased (P<0.05). There was no significant difference in the levels of PINP, CTX, and BMD between the estrogen group and the SCT treatment group (P>0.05). Conclusion SCT improves the levels of PINP and CTX, increases BMD, and inhibits bone loss in osteoporosis ovariectomized rats. It has better anti-osteoporosis effect.

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