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| sox-9通过Wnt3a/β-catenin通路促进大鼠骨髓间充质干细胞分化 |
| Sox-9 promotes differentiation of rat bone marrow mesenchymal stem cells by inhibiting Wnt3a/β-catenin pathway |
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| DOI：10.3969/j.issn.1006-7108.2021.08.014 |
| 中文关键词: 骨髓间充质干细胞 Wnt3a/β-catenin sox-9 软骨分化能力 大鼠 |
| 英文关键词:bone marrow-derived mesenchymal stem cells Wnt3a/β-catenin sox-9 cartilage differentiation ability rat |
| 基金项目:湖北省卫生计生委面上项目（WJ2017M171) |
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| 中文摘要: |
| 目的 探讨sox-9对骨髓间充质干细胞（bone marrow-derived mesenchymal stem cells，BMSCs）增殖能力和分化能力的影响。方法 分离并鉴定BMSCs，对细胞进行sox-9过表达和敲降处理。将细胞分为对照组、过表达对照组、干扰对照组、sox-9过表达组、sox-9干扰组。CCK8法检测细胞增殖活性；免疫组化观察细胞的Ⅱ型胶原蛋白（CollageⅡ）和聚蛋白聚糖（Aggrecan）表达水平；Western blot检测细胞sox-9、Wnt3a、β-连环蛋白（β-catenin）表达水平。结果 各组细胞增殖能力无显著差异（P＞0.05）；sox-9过表达组的CollageII和Aggrecan表达显著升高，而Wnt3a和β-catenin的表达显著降低（P＜0.05）。结论 sox-9可能是调控间质干细胞向软骨细胞分化能力的关键因子，通过抑制Wnt3a/β-catenin信号途径，促进其向软骨细胞分化。 |
| 英文摘要: |
| Objective To investigate the effect of Sox-9 on the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs). Methods BMSCs were isolated and identified. Sox-9 was over-expressed or knocked down in the cells. The cells were divided into blank control group, over-expression control group, interference control group, Sox-9 over-expression group, and Sox-9 interference group. MTT method was used to measure the cell growth. Immunohistochemical method was used to detect the expressions of collage II and aggrecan. Western blotting was used to detect the expressions of Sox-9, Wnt3a, and β-catenin. Results There was no significant difference in cell proliferation among the groups (P>0.05). The expression of collage II and aggrecan was significantly increased in Sox-9 over-expression group, while the expression of Wnt3a and β-catenin was significantly decreased (P<0.05). Conclusion Sox-9 may be a key factor in regulating the differentiation ability of BMSCs to chondrocytes. It promotes the differentiation of BMSCs to chondrocytes by inhibiting Wnt3a/β-catenin signaling pathway. |
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